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Project timeline and methodology

July/August 2023 – Project promotion
  • Call out to Citizen Scientists in local print and social media.
August/September 2023 – Project registration and eDNA sampling site nomination
  • Participants were asked to register an expression of interest in being a Creeks & Critters citizen scientist and provide relevant details via a Hornsby Council Yoursay project webpage.
  • Participants were asked to nominate a specific creek, dam or waterhole location (where they would like to sample for eDNA) by placing a pin on an online mapping tool and providing a relevant comment. Most proposed sampling locations were either a participant’s local creek or a favoured bushwalking spot. Some participants also shared local knowledge of their preferred location.
September/October 2023 – Finalise eDNA sampling site locations
  • The project team and scientific experts reviewed 91 creek sampling site locations submitted as part of the nomination process.
  • Based on a selection criterion, 40 sampling sites spread across Hornsby Shire were selected.
    • All sites selected were either a freshwater creek, waterhole, or dam within Hornsby LGA.
    • Creekline sampling sites were located downstream of upper catchment stormwater pipes to maximise natural habitat sampling.
    • Participants were informed that the project budget allows for the laboratory analysis of 40 eDNA samples in Spring 2023 and 40 eDNA samples in Autumn 2024. For this project, the significant cost of eDNA technology currently equates to approximately $500/site.
October 2023 – SPRING eDNA training workshops and field sampling Saturdays
  • The project requires each citizen scientist to undertake a short, in person training workshop to learn how to use an eDNA sampling kit and to be available to collect water samples on sampling Saturday field days. Workshops include a presentation from a local platypus expert and eDNA sampling kit pick-up.
  • Workshop locations and dates
    • Hornsby Library – Thursday 5 October
    • Galston Community Centre – Saturday 14 October
  • Sampling Saturdays include a base camp scenario where project team members are on hand for eDNA sampling kit pick-up/drop-off and general assistance.
    • Hornsby Council Chambers (Federation Room) – Saturday 7 October
    • Galston Community Centre – Saturday 14 October
April 2024 – AUTUMN eDNA training workshop and field sampling Saturdays
  • Saturday 6 April – eDNA Sampling Training Workshop and Sampling Saturday (Base camp at Hornsby Council Chambers)
  • Saturday 13 April – Sampling Saturday (Base camp at Galston Community Centre)
July 2024 – FINAL PROJECT PRESENTATION
  • Thursday, 25 July 2024, 6.30pm – Hornsby Library (to be confirmed)